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Meristem culture of Brazilian soybean cultivars

Annette Droste^I; Maria Helena Bodanese-Zanettini^I; Ching-yeh Hue^II

^IDepartamento de Genética, Caixa Postal 15053, Universidade Federal do Rio Grande do Sul, 91501-970, Porto Alegre, RS, Brasil. Send correspondence to M.H.B.-Z.
^IICenter for Applied Science, William Paterson College, Wayne, N.J., USA

ABSTRACT

Primary leaf-less meristem explants of Brazilian soybean cultivar "IAS-5" were excised and cultured in an organic-enriched culture establislunent medium. Ninety-five percent of the explants developed into microscopic-sized, three-budded structures within two weeks. Further incubation in the bud development medium led to the production of macroscopic multiple-budded structures in 81.9% of the cultures. The larger multiple-budded clusters were subdivided and the bud number subsequently increased in a bud multiplication medium. Some of these buds elongated and developed into plantlets which were then transplanted into soil.

Keywords: Meristem culture; Soybean.

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