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ARS activity in the yeast mitochondrial genome of low-complexity petite mutants

Daniel Delouya^I; Francisco G. Nóbrega^II

^IDepartamento de Imunologia, Instituto de Ciências Biomédicas
^IIDepartamento de Biologia, Instituto de Biociências, Universidade de Sao Paulo, Caixa Postal 11461, 05422-970 Sao Paulo, SP, Brasil. Scud correspondence to F.G.N.

ABSTRACT

The reiterated fragments of five low-complexity rho^- genomes, mapped in the apocylochrome b region of mitochondrial DNA from Saccharomyces cerevisiae, were clotted in the integrative yeast vector YIp5, yielding eight different constructions. Their capacity for autonomous replication (ARS) was subsequently assayed in yeast. One repeat unit from four genomes was sufficient to confer an ARS positive phenotype but only two repeat units from one of them conferred activity. The results support the hypothesis that the ARS 11-nucleotide sequence motif could be the cis-active region responsible for the initiation of DNA replication in rho^- genomes devoid of the putative canonical on sequences.

Keywords: Yeast; Mitochondrial genome.

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