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Juvenile hormone action on polyploidization of Apis mellifera fat body

 

 

M.M.G. BitondiI; Z.L.P. SimõesI; R.B.R de AlmeidaII

IDepartamento de Biologia, Faculdade de Filosofia, Ciéncias e Letras - USP, 14049-901 Ribeirão Preto, SP, Brasil. Send correspondence to M.M.G.B.
IIDepartamento de Genética, Faculdade de Medicina-USP, 14049-900 Ribeirão Preto, SP, Brasil

 

 

ABSTRACT

The DNA content of fat body nuclei of Apis mellifera was assayed by scanning micro-spectrophotometry, after Feulgen staining. Several classes of nuclear ploidy were found in queen fat bodies. Polyploidy in drones was lower than in queens but higher than in workers.
The competence of Apis mellifera to synthesize DNA in response to juvenile hormone was analysed. An increase in ploidy was observed in workers (newly emerged adults) after topical application of Juvenile hormone III to 5th-instar larvae (L5F).

Keywords: Juvenile hormone; Polyploidization; Apis mellifera; Fat body.

 

 

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