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Genetic recombination after protoplast fusion in Candida sp.

Maria Helena Pelegrinelli Fungaro^I; João Lúcio de Azevedo^II; Aline Aparecida Pizzirani-Kleiner^II

^IDepartamento de Biologia, CCB, Universidade Estadual de Londrina, 86051 Londrina, PR, Brasil. Send correspondence to M.H.P.F.
^IIDepartamento de Genética, ESALQ-USP, Caixa Postal 83, 13400 Piracicaba, SP, Brasil

ABSTRACT

The objective of this work was to describe the parasexual cycle of Candida sp, which is generally considered to exist in the asexual state. The conditions for protoplast isolation and regeneration were established for the wild-type strain of Candida sp. Protoplasts prepared from the auxotrophic strains (pab bio and um arg) were fused with polyethylene glycol. Prototrophic derivatives formed by this fusion protocol were selected by complementation on minimal medium. From a selected nuclear fusion product, several monoauxotrophic recombinants were obtained, whose nuclear diameter remained similar to the parental ones. This fact suggested that the auxotrophic recombinants arose from chromosome loss.

Keywords: Genetic recombination; Protoplast fusion; Candida sp.

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