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Isolation of cellulase-producing mutants from a Penicillium sp strain denominated 3MUV24

Aldo J.P. Dillon; Suelen O. Paesi-Toresan; Luciana P. Barp

Departamento de Ciéncias Biológicas, Instituto de Biotecnologia, Universidade de Caxias do Sul, Rua Francisco Getúlio Vargas, 1130, Caixa Postal 1352, 95070 Caxias do Sul, RS, Brasil

ABSTRACT

Three cellulase producing mutants were obtained from Penicillium sp strain 3MUV24. One of them arose spontaneously and the other two were obtained by treatment with UV-light (254 nm). Selection was performed on agar plates containing cellulose. Mutants were detected on the basis of production of large clear areas on the cellulose agar plates. The filter paper hydrolyzing activity and b-glucosidase production by one of the mutant strains were approximately two-fold and six-fold, respectively, when compared to the parental strain.

Keywords: Cellulase; Penicillium sp.

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