Full text in pdf format

The effects of aDp (II-I) in the meiotic recombination of Aspergillus nidulans

José Moacir Marin^I; Tânia M.A. Domingues Zucchi^II

^IDepartamento de Ciências Morfológicas, Faculdade de Odontologia, USP, 14049 Ribeirão Preto, SP, Brasil
^IIDepartamento de Parasitologia, Instituto de Ciências Biomédicas, USP, Av. Prof. Linen Prestes, 1374, 05508 São Paulo, SP, Brasil. Send correspondence to T.M.A.D.Z.

ABSTRACT

This paper reports the effects of Dp (II-I) on the meiotic recombination of an Aspergillus nidulans mutant. The mutation involves a non reciprocal translocation of a segment bearing a duplication of the gene meth⁺ from linkage group II to I. The insertion of this segment occurs in the paba A₁₂₄ - y A₂ interval of chromosome I, promoting several genic rearrangements which alter the meiotic behavior of the genetic markers adjacent to the insertion point. The implications of the recombination when the Dp (II-I) is spontaneously lost by deletion or by crossing-over were also studied.

Keywords: Meiotic; Aspergillus nidulans.

REFERENCES

Azevedo, J.L. (1975). Altered instability due to genetic changes in a duplication strain of Aspergillus nidulans. Genet. Res. 26: 55-61.

Azevedo, J.L.and Roper, J.A. (1970). Mitotic non conformity in Aspergillus: successive and transposable genetic changes. Genet. Res. 16: 79-93.

Bainbridge, B.W. and Roper, J.A. (1966). Observations on the effects of a chromosome duplication in Aspergillus nidulans. J. Gen. Microbiol. 42: 417-424.

Ball, C. (1966). Instability associated with chromosome translocation inAspergillus nidulans. Heredity 21: 531.

Borts, R.H., Lichten, M., Hearn, M., Dowidow, L.S. and Haber, J.E. (1984). Physical monitoring of meiotic recombination in Saccharomyces cerevisiae. Cold Spring Harbor Symposia on Quantitative Biology 49: 67-76.

Burr, K.W., Roper, J.A. and Relton, J. (1982). Modification of chromosome instability in Aspergillus nidulans. J. Gen. Microbiol. 128: 2899-2907.

Castro Prado, M.A.A. (1986). Estabilização de genes em dupla dose entre alguns mutantes hiper-rec-uvs de Aspergillus nidulans. Master's Thesis, USP, Ribeirão Preto.

* Castro Prado, M.A.A. and Zucchi, T.M.A.D. (1991). Meiotic segregation of a recessive gene (w A₂) included in a Dp (II-I) of Aspergillus nidulans. Rev. Bras. Genet. 14: 249-260.

* Castro Prado, M.A.A. and Zucchi, T.M.A.D. (1991). Stabilization of a duplicated segment Dp (II-I) in an uvs mutant of Aspergillus nidulans through genetic mechanisms. Rev. Bras. Genet. 14: 239-248.

Dellaporta, S.L., Chamet, P.S., Mottinger, J.P., Woods, J.A., Yu, S.M. and Hicks, J.B. (1984). Endogenous transposable elements associated with virus infection in maize. Cold Spring Harbor Symposia on Quantitative Biology 49: 321-328.

Majerfeld, I.H. and Roper, J.A. (1978). The effects of coumarin on the frequency of deletions in a duplication strain of Aspergillus nidulans. Mol. Gen. Genet. 159: 203-206.

Marin, J.M. (1983). Estudo de fatores genéticos que afetam a estabilidade mitótica e recombinação em Aspergillus nidulans. Master's Thesis, USP, Ribeirão Preto.

* Marin, J.M. and Zucchi, T.M.A.D. (1991). Genetic analysis of some factors affecting mitotic and meiotic behavior of a mutant of Aspergillus nidulans. Rev. Bras. Genet. 14: 9-20.

Nevers, P. and Saedler, H. (1977). Transposable genetic elements as agents of gene instability and chromosomal rearrangements. Nature 268: 109-115.

Nga, B.H. and Roper, J.A. (1968). Quantitative intrachromosomal changes arising at mitosis in Aspergillus nidulans. Genetics 58: 193-209.

Pontecorvo, G., Roper, J.A., Hemmons, L.M., McDonald, K.D. and Bufton, A.W.J. (1953). The genetics of Aspergillus nidulans. Adv. Genet. 5: 141-238.

Van de Vate, C. and Jansen, G.J.O. (1978). Meiotic recombination in a duplication strain of Aspergillus nidulans. Genet. Res. 31: 29-52.

Zucchi, T.M.A.D. (1986). Estudos de fatores genéticos que afetam as frequências de recombinação em Aspergillus nidulans (EIDAM) WINTER. Associate Professor's Thesis, USP, Ribeirão Preto.

* Zucchi, T.M.A.D. (1990). Isolation of putative recombination mutants of Aspergillus nidulans. Rev. Bras. Genet. 13: 409-424.

Zucchi, T.M.A.D. and Azevedo, J.L. (1979). Mitotic instability in a III-VIII duplications strain of Aspergillus nidulans. Rev. Bras. Genet. 2: 93-108.