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Alu I and hae III restriction endonucleases, human chromosomes and infertility

 

 

Guillermo Barreto; Francisco M. Salzano

Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Caixa Postal 15053, 91501 Porto Alegre, RS, Brasil. Send correspondence to F.M.S.

 

 

ABSTRACT

The Alu I and Hae III bands of twenty-five couples plus two isolated members of two other couples with reproductive problems, as well as the same number of appropriate controls were studied. Both qualitative and quantitative (densitometric) approaches were employed. Chromosome 1 was the most heteromorphic for the two enzymes, but others also showed heteromorphism frequencies equal or higher than 10% (Alu I: chromosomes 3, 4, 9, 15, 16, 19 and 22; Hae III: 16). The Alu I bands of chromosomes 9, 15, 19 and 22 were measured densitometrically. Those of chromosome 9 were larger than those observed in the other chromosomes, and those of chromosome 19 larger than those of chromosome 22. The heteromorphism indices proved to be strikingly similar. Agreement between que qualitative and quantitative approaches was tested and found to be satisfactory. No significant differences were observed between the infertile and fertile individuals.

Keywords: Alu 1; HAE; endonucleases, human chromosomes; infertility.

 

 

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