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Biology of Amazonian anopheline mosquitoes. XVI evidence of multiple insemination (Poliandry) in Anopheles nuneztovari gabaldon, 1940 (Diptera: Culicidae)

Vera M. Scarpassa^I; Wanderli P. Tadei^II; Warwick E. Kerr^III

^IDepartamento de Ciências da Saúde, INPA, Caixa Postal 478, 69083 Manaus, AM, Brasil. Send correspondence to V.M.S.
^IIDepartamento de Ciências da Saúde, INPA, Caixa Postal 478, 69083 Manaus, AM, Brasil./UNESP-SJRP, SP
^IIIDepartamento de Biociências, Universidade Federal de Uberlândia, Caixa Postal 593, Campus de Umuarama, 38400 Uberlândia, MG, Brasil

ABSTRACT

Genetic segregation among the descendants of Anopheles nuneztovari for the isoalleles EST1 *F, EST1 *S; EST2*F, EST2*S; EST6*F, EST6*M, EST6*S were analyzed in our laboratories. These analyses indicated that effective multiple insemination (poliandry) occurs in natural populations of A. nuneztovari. 40 females were collected in the field, and eight descendants per female were submitted to electrophoresis. The results indicated that at least 15% of the females had semen from two males and the remaining 85% had brood fathered by a single male.

Keywords: Polyandry; Anopheles nuneztovari; Diptera.

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