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Quantitative differences between human red cell esterase D phenotypes

M.N. Klautau-Guimarães^I; M.A. Mestriner^I; H. Krieger^II

^IDepartamento de Genética, Faculdade de Medicina de Ribeirão Preto-USP, 14049 Ribeirão Preto, SP, Brasil. Send correspondence to M.N.K.-G.
^IIDepartamento de Genética, FIOCRUZ, Av. Brasil, 4365, 20010 Rio de Janeiro, RJ, Brasil

ABSTRACT

Red cell ESD enzyme activity was determined in 125 ESD1 phenotype individuals, 69 ESD2-1 individuals and 9 ESD2 individuals. Mean activity for the three groups was 265.04, 230.35 and 161.66 units, respectively, a unit being defined as the amount of enzyme capable of hydrolyzing 10^-7 mol 4-methylumbelliferyl acetate/hour/g hemoglobin (Hb).
The distribution of enzyme activity in the sample appeared to be unimodal, with significant variability among phenotypes. The activity associated with the ESD*1 allele was estimated to be 60% higher than that associated with the ESD *2 allele, this difference accounting for 15% of the total quantitative variation of red cell ESD in this sample.

Keywords: Human red cell; Esterase D.

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