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Preparation and regeneration of protoplasts of Talaromyces flavus

T.M.C. Santos^I; I.S. De Melo^II

^ICentro de Energia Nuclear na Agricultura, CENA/USP, 13400 Piracicaba, SP, Brasil
^IICentro Nacional de Pesquisa de Defesa da Agricultura, CNPDA/EMBRAPA, Caixa Postal 69, 13820 Jaguariúna, SP, Brasil. Send correspondence to I.S.M.

ABSTRACT

Protoplasts from Talaromyces fiavus were produced from young mycelia using the lytic enzyme, Novozym 234. The best culture medium for highest mycelia yield was malt extract with 20 hours of incubation. The osmotic stabilizers Glucose, NaCI and KCI in regeneration medium were found to induce high regeneration of protoplasts, such as 65%, 65.75% and 70.75%, respectively. The rate of regeneration with (NH4)2SO4 was 4.5%.

Keywords: Regeneration; Protoplasm; Talaromyces flavus.

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