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The cytogenetics of Abracris flavolineata (orthoptera, Caelifera, Ommatolampinae, Abracrini)

Doralice Maria Cella; Amilton Ferreira

Departamento de Biologia, Instituto de Biociências, UNESP, Caixa Postal 178, 13500 Rio Claro SP, Brasil

ABSTRACT

The mitotic (neuroblastic) and meiotic (testicular) chromosomes of a population from Rio Claro of Abracris flavolineata were analysed by standard (Giemsa) and differential (C banding, Mild N, Strong N and silver) staining. All of the chromosomes in the karyotype (2nd = 23 and 2n 4 = 24) differed in morphology from those of the basic karyotype of the superfamily Acridoidea, all of which are acrocentric. The chromosomes of pairs L1 - L3, M4 - M6 and M8 were subtelocentric, those of pairs M7 and S9 were submetacentric, and those of S10 and S11 metacentric. The sex chromosome was subtelocentric. C banding showed that the small arms of all chromosomes except those of pair M7 originated from the addition of chromosomal material made up of constitutive chromatin. The submetacentric morphology of pair M7 originated from a pericentric inversion. Silver staining did not reveal any region with the characteristics of a NOR but permitted the visualization of points similar to Cd bands in the centromeric regions, which were interpreted to be kinetochrore plates. N banding stained the proximal region of the long arms of the chromosomes of pair M8, which was considered to be one of the nucleolar organizer regions of A. flavolineata.

Keywords: Cytogenetics; Abracris flavolineata; Orthoptera.

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