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Comparative study of puffing patterns of Drosophila willistoni and Drosophila cubana salivary gland chromosomes

Vera Lucia S. Valente^I; Antonio Rodrigues Cordeiro^II

^IDepartamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Caixa Postal 15053, 91501 Porto Alegre, RS, Brasil. Send correspondence to V.L.S.V.
^IIDepartamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Caixa Postal 68011, Cidade Universitária, 20000 Rio de Janeiro, RJ, Brasil

ABSTRACT

The puffing patterns of the 3^rd salivary gland chromosomes of Drosophila willistoni and Drosophila cubana were studied in synchronized third instar larvae, in zero hour prepupae, and at 1, 2, 4 and 6 hour intervals after anterior spiracle eversion. All bands observable in the optical microscope (1000 x) were scored, and a total of 277 bands or puffs were identified in chromosome three of D. willistoni, while for D. cubana, 247 were detected. The puffing pattern of 103 and 56 bands showed median size 2 puffs in at least one stage in D. willistoni and D. cubana, respectively. However, D. willistoni puffing activity decreased steadily after two hours of puparium formation and D. cubana showed an increase of average activity until six hours.
Our data suggest that if changes in gene regulation maybe evaluated through puffing patterns, these changes must have been very frequent in the species studied.

Keywords: Puffing patterns; Drosophila willistoni; Drosophila cubana; chromosomes.

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