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Electrophoretic analysis of 12 proteins in natural populations of Spodoptera frugiperda (Lepidoptera: Noctuidae)^*

Leda M.K. Sodré de Lima^I; Eucléia P.B. Contel^II

^IDepartamento de Biologia Geral, Universidade Estadual de Londrina, 86051 Londrina, PR, Brasil. Send correspondence to L.M.KS.L.
^IIDepartamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049 Ribeirão Preto, SP, Brasil

ABSTRACT

Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae) is considered to be one of the most serious pests for crops such as corn, rice and other Gramineae, and its control is of great economic importance.
Studies on this species have mainly focussed on the agricultural damage caused by the pest and on its control. There are practically no studies on genetic variability at the protein level within and among S. frugiperda populations.
The objective of the present study was to determine the electrophoretic patterns of twelve proteins (peptidases, carboxylesterases, malic enzyme, glucose dehydrogenase, alcohol dehydrogenase, glycerol-3-phosphate dehydrogenase, isocitrate dehydrogenase, leucine-aminopeptidase, nonspecific proteins, phosphoglucomutase, malate dehydrogenase, and superoxide dismutase) in natural S. frugiperda populations, as well as the number of loci involved, and to identify possible allele variants.
Six of the twelve proteins studied (malic enzyme, glucose dehydrogenase, alcohol dehydrogenase, peptidase, superoxide dismutase, and malate dehydrogenase) are described for the first time for this species. Twenty-two loci were detected, 13 of which presented genetically determined variation. Statistical analysis of the population data is in preparation.

Keywords: Electrophoretic; Spodoptera frugiperda; Lepidoptera.

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* Part of a thesis presented by L.M.K.S.L. to the Departamento de Genética, FMRP, USP in partial fulfillment of the requirements for the Doctoral degree.