Full text in pdf format

Genetic study of a triadimefon-resistant mutant of Aspergillus nidulans

Sandra Regina Campos Pombeiro; Nilce Maria Martinez-Rossi

Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, USP, 14049 Ribeirão Preto, SP. Brasil. Send correspondence to N.M.M.-R.

ABSTRACT

The trdA9 gene was identified in Aspergillus nidulans which confers resistance to the fungicides triadimefon and imazalil. This gene mapped in linkage group VII, is probably an alelle to imaA4, and behaved as a dominant character. Although the degree of resistance shown by the mutant strain is relatively low, it was suggested that the mutant may be useful for the development of a DNA transformation system in A. nidulans. Also it seems that triadimefon does not induce point mutations, in the methionine system of A. nidulans.

Keywords: Triadimefon-resistant mutant; Aspergillus nidulans.

REFERENCES

Bellincampi, D., Gualandi, G., La Monica, E., Poley, C. and Morpurgo, G.P. (1980). Membrane-damaging agents cause mitotic non-disjunction in Aspergillus nidulans. Mutation Res. 79: 169-172.

Buchenauer, H. (1977). Mode of action of triadimefon in Ustilago avenae. Pestic. Biochem. Physiol. 7: 309-320.

Burden, RS., Cooke, D.T. and Carter, GA. (1989). Inhibitors of sterol biosynthesis and growth in plants and fungi. Phytochem. 28: 1791-1804.

Erickson, J.M., Rahire, M. and Rochaix, J.D. (1985). Herbicide resistance and cross-resistance: Changes at three distinct sites in the herbicide-binding protein. Science 228: 204-207.

Fernández-Larrea, J. and Stahl, U. (1989). Transformation of Podospora anserina with a dominant resistance gene. Curr. Genet. 16: 57-60.

Forbes, E. (1959). Use of mitotic segregation for assigning genes to linkage groups in Aspergillus nidulans. Heredity 13: 67-80.

Gabridge, M.G. and Legator, M.S. (1969). A host-mediated microbial assay for the detection of mutagenic compounds. Proc. Soc. Exp. Biol. Med. 130: 831-834.

Hippe, S. (1984a). Ultrastructural changes induced by the systemic fungicides triadimefon, nuarimol and imazalil nitrate in sporidia of Ustilago avenae. Pestic. Sei. 15: 210-214.

Hippe, S. (1984b). Electron microscopy investigations on the cytology of Ustilago avenae as influenced by the systemic fungicides triadimefon, nuarimol and imazalil-nitrate: a thin-section and freeze-fracture study. Pestic. Biochem. Physiol. 21: 120-183.

Hippe, S. and Niedermeyer, W. (1984). Freeze-fracture electron microscopy of the plasmalemma of Ustilago avenae treated with systemic fungicides: a quantitative analysis of the intramembrane particles. Pestic. Biochem. Physiol. 21: 184-193.

Leroux, P. and Gredt, M. (1976). Comparaisons des modes d'action fongitoxique du triadiméfon (Meb6447), du triarimol et de la triforine. Phytopath. Z. 86: 276-279.

Lhoas, P. (1961). Mitotic haploidization by treatment of Aspergillus niger diploids with pfluorophenylalanine. Nature (London) 190: 744.

Lilly, L.J. (1965). An investigation of the suitability of the suppressors of meth-1 in Aspergillus nidulans for the study of induced and spontaneous mutation. Mutation Res 2: 192-195.

Martinez-Rossi, N.M. and Azevedo, J.L. (1987). Detection of point-mutation mutagens in Aspergillus nidulans: comparison of methionine suppressors and arginine resistance induction by fungicides. Mutation Res. 176: 29-35.

McCully, K.S. and Forbes, E. (1965). The use ofp-fluorophenylalanine with "master strains" of Aspergillus nidulans for assigning genes to linkage groups. Genet. Res. 6: 352-359.

Morpurgo, G. (1961). Somatic segregation induced byp-fluorophenylalanine.Aspergillus Newsl. 2: 10.

Pontecorvo, G., Roper, J.A., Hemmons, L.M., MacDonald, K.D. and Bufton, A.W.J. (1953). The genetics of Aspergillus nidulans. Adv. Genet. 5: 141-238.

Richmond, D.V. (1984). Effects of triadimefon on the fine structure of germinating conidia of Botrytis allii. Pestic. Biochem. Physiol. 21: 74-83.

Roper, JA. (1952). Production of heterozygous diploids in filamentous fungi. Experientia 8: 14-15.

Saag, M.S. and Dismukes, W.E. (1988). Azole antifungal agents: emphasis on new triazoles.Antimicrob. Agents Chemother. 32: 1-8.

Sherald, J.L. and Sisler, H.D. (1975). Antifungal mode of action triforine. Pestic. Biochem. Physiol. 5: 477-488.

Siddiqi, O.H. (1962). Mutagenic action of nitrous acid on Aspergillus nidulans. Genet. Res. 3: 303-314.

Speakman, J.B. and Nirenberg, H.I. (1981). Mutagenicity of methyl benzimidazol-2-yl carbamate (MBC) towards Aspergillus nidulans (Eidam) Winter and Cladosporium cucumerinum Ellis & Arth. Mutation Res. 88: 45-51.

Stiers, D.L., Fellman, J.K. and Tourneau, D.L. (1980). The effects of triadimefon on growth, sclerotial formation, and ultrastructure of Sclerotinia scierotiorum. Environm. Experim. Botany 20: 181-189.

Tuyl, J.M. van (1977). Genetics of fungal resistance to systemic fungicides. Mendedelingen Landbouwhogeschool Wageningen (Nederl.) 77(2): 1-136.

Waard, M.A. and Sisler, H.D. (1976). Resistance to fenarimol in Aspergillus nidulans. Meded. Fac. Landbouwwet. Rijksuniv. Gent. 41: 571.