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Parasexual analysis of Aspergillus awamori by using intraspecific diploids and intraspecific hybrids with A. niger

Airton Vialta^I; Renato Bonatelli Junior^II

^ICODETEC, Caixa Postal 6041, 13081 Campinas, SP, Brasil
^IIDepartamento de Genética e Evolução, Instituto de Biologia, UNICAMP, Caixa Postal 6109, 13081 Campinas, SP, Brasil. Send correspondence to R.B.Jr.

ABSTRACT

Auxotrophic, morphological and resistant mutants have been isolated from Aspergillus awamori NRRL 3112 strain and used for conventional genetical analysis by the parasexual cycle. A minimum of four linkage groups was suggested for that species. Also interspecific hybrids with A. niger were obtained only afterprotoplast fusion and segregants from them suggested: I) that genetical analysis should be done as there is confirmation of linkage groups previously determined with intraspecies diploids; II) new linkage relationships between awamori markers and III) homology of at least one linkage group on both species that allowed mitotic crossing over to occur. These suggestions are considered as indications of a very close phylogenetical relationship between these species.

Keywords: Parasexual; Aspergillus awamori; A. niger.

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