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Mutation response of a diploid pso4-1 yeast strain defective in error-prone recombinational DNA repai

K.V.C.L. da Silva^I; H.H.R. de Andrade^II; J.A.P. Henriques^I

^IDepartamento de Fisiologia, Farmacologia e Biofísica, Setor de Biofísica, Instituto de Biociências, UFRGS, Rua Sarmento Leite, 500, 90049 Porto Alegre, RS, Brasil. Send correspondence to K.V.C.L.S.
^IIDepartamento de Genética, Instituto de Biociências, UFRGS, Rua Prof. Annes Dias, 112, 15° andar, 90020 Porto Alegre, RS, Brasil

ABSTRACT

The mutagenic response of diploid Saccharomyces cerevisiae cells homozygous for the pso4-1 mutation was compared to that of the corresponding wild-type strain. The pso4-1 diploid presented a marked reduction in the reverse mutation frequencies induced by 254 nm-UV radiation or by mono- and bifunctional furocoumarin photoaddition. For these different agents, the frequency of revertants was reduced within a range of high doses. It has been previously shown that, in addition to affecting the mutagenesis response, the pso4-1 mutant is completely defective in both reciprocal (crossing-over) and non-reciprocal (gene conversion) mitotic recombination. These data indicate that the PSO4 gene simultaneously controls the mutagenesis and recombination processes by acting on an error-prone recombinational repair pathway comparable to the SOS repair present in Escherichia coll.

Keywords: Pso4-1 yeast; Error-prone; Recombinational; DNA repair.

REFERENCES

Andrade, H.H.R., Marques, E.K., Schenberg, A.C.G., Henriques, J.A.P. (1989). The PSO4 gene is responsible for an error-prone recombinational DNA repair pathway in Saccharomyces cerevisiae. Mol. Gen. Genet. 217: 419-426.

Averbeck, D. (1982). Photobiology of furocumarins. In: Trends in Photobiology (Helene, C., Charlier, M., Montenay-Ganestier, Th. and Laustriat, G., eds.). Plenum, New York, pp. 295-308.

Averbeck, D. and Moustacchi, E. (1975). 8-Metoxypsoralen plus 365 nm light effects and repair in yeast. Biochim. Biophys. Acta, 395: 393-404.

Averbeck, D. and Moustacchi, E. (1979). Genetic effects of 3-carbethoxypsoralen, psoralen and 8-methoxypsoralen plus 365 nm irradiation in saccharomyces cerevisiae. Induction of reversions, mitotic crossing-over, gene conversion and cytoplasmic "petite" mutation. Mutat. Res. 68: 133-148.

Averbeck, D., Moustacchi, E. and Bisagni, E. (1978). Biological effects and repair of damage photoinduced by a derivate of psoralen substituted at the 3,4 reaction side. I. Photoreactivity of this compound and lethal effect in yeast. Biochim. Biophys. Acta 518: 464-481.

Benathen, LA. and Beam, CA. (1977). The genetic control of X-ray resistance in budding yeast cells. Radiat. Res. 69: 99-116.

Cassier, C., Chanet, R, Henriques, J.A.P. and Moustacchi, E. (1980). The effects of three pso genes on induced mutagenesis: A novel class of mutationally defective yeast. Genetics 96: 841-857.

Cassier, C. and Moustacchi, E. (1981). Mutagenesis induced by mono and bifunctional alkylating agents in yeast mutants sensitive to photoaddition of furocumarins (pso). Mutat. Res. 84: 37-47.

Cassier-Chauvat, C. and Moustacchi, E. (1988). Allelism between pso/-1 and rev3-1 mutants and between pso2-1 and smml-1 mutants in Saccharomyces cerevisiae. Curr. Genet. 13: 37-40.

Friedberg, E.C. (1988). Deoxyribonucleic acid repair in the yeast Saccharomyces cerevisiae. Microbiol. Rev. 52: 70-102.

Game, J.C. (1983). Radiation-sensitive mutants and repair in yeast. In: Yeast Genetics, Fundamental and Applied Aspects (Spencer, J.F.T., Spencer, D.M. and Smith, A.RW., eds.). Springer-Verlag, New York, pp. 109-137.

Game, J.C., Zamb, T.J., Braun, R.J., Resnick, M.A. and Roth, R.M. (1980). The role of radiation (rad) genes in meiotic recombination in yeast. Genetics 94: 51-68.

Grant, E.L., von Borstel, R.C. and Ashwood-Smith, M.J. (1979). Mutagenicity of cross-links and monoadducts of furocumarins (psoralen and angelicin) induced by 360-nm radiation in excision-repair defective and radiation-insensitive strains of Saccharomyces cerevisiae. Environ. Mutagen. 1: 55-63.

Haynes, RH. and Kunz, B.A. (1981). DNA repair and mutagenesis in yeast. In: The molecular biology of the yeast Saccharomyces (Strathern, J.N., Jones, E.W. and Broach, J.R, eds.). Cold Spring Harbor Laboratory, New York. o. 371-414.

Henriques, J.A.P. and Moustacchi, E. (1980). Isolation and characterization of pso mutants sensitive to photo-addition of psoralen derivatives in Saccharomyces cerevisiae. Genetics 95: 372-288.

Henriques, J.A.P. and Moustacchi, E. (1981). Interaction between mutations for sensitivity to psoralen photoaddition (pso) and to radiation (rad) in Saccharomyces cerevisiae. 1. Bacteriol. 148: 248-256.

Henriques, JA.P., Da Silva, K.V.C.L. and Moustacchi, E. (1985). Interaction between genes controlling sensitivity to psoralen (pso) and to radiation (rad) after 3-carbetoxypsoralen plus 365 nm UV light treatment in yeast. Mol. Gen. Genet. 201: 415-420.

Henriques, JA.P., Andrade, H.H.R., Bankmann, M. and Brendel, M. (1989). Reassessing the genotoxic potential of 8-MOP + UVA - induced DNA damage in the yeast Saccharomyces cerevisiae. Curr. Genet. 16: 75-80.

Henriques, JA.P., Vicente, E.J., Silva, K.V.C.L. and Schenberg, A.C.G. (1989). A novel gene involved in error-prone repair in Saccharomyces cerevisiae. Mutat. Res. 218: 111-124.

Lawrence, C.W. (1982). Mutagenesis in Saccharomyces cerevisiae. In:Advances in Genetics (Caspari, E.W., ed.). Academic Press, New York, p. 173-254.

Lawrence, C.W. and Christensen, R. (1976). UV mutagenesis in radiation-sensitive strains of yeast. Genetics 82: 207-232.

Machida, I. and Nakai, S. (1980). Induction of spontaneous and UV-induced mutations during commitment of meiosis in Saccharomyces cerevisiae. Mutat. Res. 73: 59-68.

Magaña-Schwencke, N., Averbeck, D., Henriques, J.A.P. and Moustacchi, E. (1980). Absence de pontages inter-chaines dans I´traité par le 3-carbéthosypsoralene et une irradiation a 365 nm. C.R. Acad. Sci. Paris 291:207-210.

Malone, RE. and Esposito, R.E. (1980). The RAD 52 gene is requerid for homothallic interconversion of mating types and spontaneous recombination in yeast. Proc. Nat. Acad. Sci. USA 77: 503-507.

McKee, RH. and Lawrence, C.W. (1979). Genetic analysis of gamma-ray mutagenesis in yeast. I. Reversion in radiation-sensitive strains. Genetics 93: 361-373.

Montelone, B.A., Prakash, S. and Prakach, L. (1981). Recombination and mutagenesis in rad 6 mutants of Saccharomyces cerevisiae: evidence for multiple functions of the RAD6 gene. Mol. Gen. Genet. 184: 410-415.

Moustacchi, E. (1987). DNA repair in yeast: genetic control and biological consequences. In: Advances in Radiation Research. (Lett, J., ed.). Academic Press, New York, vol. 13, p. 1-30.

Munson, R.J. and Goodhead, D.T. (1977). The relation between induced mutants frequency and cell survival. A theoretical approach and an examination of experimental data for eukaryotes. Mutat. Res. 42: 145-160.

Nakai, S. and Matsumoto, S. (1967). Two types of radiation-sensitive mutant in yeast. Mutat. Res. 4: 129136.

Parsons, B.J. (1980). Psoralen photochemistry. Photochem. Photobiol. 32: 813-821.

Prakash, L. and Taillon-Miller, P. (1981). effects of the rad 52 gene on sister chromatid recombination in S. cerevisiae. Curr. genet. 2: 247-250.

Saeki, T., Cassier, C. and Moustacchi, E. (1983). Induction in Saccharomyces cerevisiae of mitotic recombination by mono and bifunctional agents: comparison of the pso2.1 and rad 52 repair deficient mutants to the wild-type. Mol. Gen. Genet. 190: 255-264.

Saeki, T., Machida, I. and Nakai, S. (1980). Genetic control of diploid recovery after y-irradiation in the yeast Saccharomyces cerevisiae. Mutat. Res. 73: 251-265.

Siede, W. and Eckardt, F. (1984). Inducibility of error-prone DNA repair in yeast? Mutat. Res. 129: 3-11.

Song, P.S. and Tapley, K.J. (1979). Photochemistry and photobiology of psoralens. Photochem. Photobiol. 29: 1177-1197.

Wilcox, D.R. and Prakash, L. (1981). Incision and postincision steps of pyrimidine dimer removal in excision - defective mutants of Saccharomyces cerevisiae. J.. Bacteria 148: 618-623.