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Effect of pulsed ultrasound on the frequency of SCE and on cell proliferation

N. Ameida-Melo^I; I. Ferrari^II; L.R. Martelli^III; J.H.P. Paulim^IV

^IDepartamento de Bioagressão, Instituto de Ciências da Saúde, Universidade Federal da Bahia, 40000 Salvador, Bahia, Brasil. Send correspondence to N.A.-M.
^IIDepartamento de Medicina Especializada, Faculdade de Ciências da Saúde, Laboratório de Genética Clínica, Universidade Nacional de Brasília, 70254 Brasília, DF, Brasil
^IIIDepartamento de Genética e Matemática Aplicada à Biologia, Faculdade de Medicina de Ribeirão Preto, USP, 14049 Ribeirão Preto, SP, Brasil
^IVDepartamento de Bioengenharia, Faculdade de Medicina de Ribeirão Preto, USP, 14049 Ribeirão Preto, SP, Brasil

ABSTRACT

The effects of chronic and acute ultrasound doses on lymphocytes obtained from three males and three females were studied in terms of sister chromatid exchange and cell proliferation index during the various phases of the cell cycle. A decrease in the frequency of M₂ cells in 48-hour cultures (control = 1.01; G₁ = 0.48; S = 0.29; 1% < p < 5%) treated with chronic doses of ultrasound and the absence of these cells in cultures treated with acute doses recommends caution in the use of ultrasound waves for therapeutic purposes. Culture time (42 and 72 hours) had no effect on the frequency of sister chromatid exchange either in the ultrasound-treated group or the control.

Keywords: Pulsed ultrasound; SCE; Cell proliferation.

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