Full text in pdf format

Comparison of natural variants of the soybean cultivar parana by isoenzyme analysis

Eric Derbyshire^I; M. Teresa V. Carvalho^I; Emídio R. Bonato^II

^ICentro de Energia Nuclear na Agricultura (CENA), Universidade de São Paulo, Caixa Postal 96, 13400 Piracicaba, SP, Brasil. Send correspondence to E.D.
^IICentro Nacional de Pesquisa de Trigo - EMBRAPA, Caixa Postal 569, 99001 Passo Fundo, RS, Brasil

ABSTRACT

The natural variants of the soybean cultivar Paraná, Paranagoiana, SS-1 and Pirapó-78, are considered mutants of the original cultivar. However the morphology of cultivar Pirapó-78 raises doubsts about its origin through mutation. To clarify this point the electrophoretic profiles of the total protein, acid phosphatase, alcohol dehydrogenase, esterase, malic dehydrogenase, peroxidase and urease of seed extracts of the four cultivars were determined. These did not permit the cultivars Paranagoiana and SS-1 to be distinguished from the original cultivar. However peroxidase, esterase and malic dehydrogenase profiles of cultivar Pirapó-78 were different from the respective profiles of the other cultivars. The data are consistent with origin of cultivars Paranagoiana and SS-1 by mutation but suggest a different origin for Pirapó-78.

Keywords: Soybean; Isoenzyme.

REFERENCES

Brewbaker, J.L., Upadhya, M.D., Makinen, Y. and MacDonald, T. (1968). Isoenzyme polymorphism in flowering plants. III - Gel electrophoretic methods and applications. Physyologia Plantarum 21: 930940.

Buttery, B.R. and Buzzell, RI. (1968). Peroxidase activity in seeds of soybean varieties. Crop. Sci. 8: 722725.

Buttery, B.R. and Buzzell, RI. (1971). Properties and inheritance of urease isoenzymes in soybean seeds. Can. J. Bot. 49: 1101-1105.

Cedeño, O.G. and Perez, J.E. (1985). Caracterizacion de doce cultivares de soya (Glycine max [L] Men.) por métodos bioquímicos. Rev. Fac. Agron. (Maracay) XIV 67-79.

Davis, B.J. (1964). Disc electrophoresis. II. Method and applications to human serum proteins. Ann. N.Y. Acad. Sci. 121: 407-427.

Doong, J.Y.H. and Kiang, Y.T. (1987). Cultivar identification by isozyme analysis. Soybean Genetics Newsletter 14: 189-226.

Kiang, Y.T. and Gorman, M.B. (1983). Soybean. In: Isoenzymes in plant genetics and breeding (Tanksley, S.D. and Orton, TJ. eds.) Elsevier Science Publishing Company, Inc. New York, pp. 295-328.

Kiihl, R.A.S., Costa, A.V., Bays, I.A., Almeida, L.A. and Garcia, A. (1984). Cultivar de soja Paranagoiana. In: Seminario Nacional de Pesquisa de Soja. 3 Anais, EMBRAPA/CNPS, Campinas, pp. 473-474.

Larsen, A.L. (1967). Electrophoretic differences in seed proteins among varieties of soybean, Glycine max (L.) Merril. Crop. Sic. 7: 311-313.

OCEPAR/EMBRAPA (1988). Recomendações para a cultura da soja no Paraná, 1988/1989. Boletim Técnico 23, Cascavél, Paraná.

Shaw, C.R. and Koen, A.L. (1964). Aspartate dehydrogenase activity of malate dehydrogenase. Biochem. Biophys. Acta 92: 397-401.

Shaw, C.R. and Koen, A.L. (1965). On the identity of "nothing dehydrogeanse". J. Histochem. Cytochem. 13: 431-435.

Shaw, C.R and Prasad, R (1970). Starch gel electrophoresis of enzymes. A compilation of recipes. Biochem. Gen. 4: 297-320.

Tanksley, S.D. and Orton, TJ. (1983). Isoenzymes in plant genetics and breeding. Elsevier Science Publishing Company Inc, New York.