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Sozyme extraction from mature OIL Palm (Elaeis guineensis, Jacq.) leaves for electrophoretic studies

Marcio de Miranda Santos^I; Moacyr Antônio Mestriner^II

^IEMBRAPA, Centro Nacional de Pesquisa de Seringueira e Dendê, Caixa Postal 319, 69000 Manaus, AM, Brasil. Send correspondence to M.M.S.
^IIDepartamento de Genética e Matemática Aplicada à Biologia, Faculdade de Medicina de Ribeirão Preto, USP, 14049 Ribeirão Preto, SP, Brasil

ABSTRACT

The oxidation of phenolics, due to the action of the phenoloxidase complex, to form quinones and tannins is commonly observed during extraction of plant isozymes and organelles. These products are powerful enzymatic inhibitors. To overcome such a process when working with mature Oil Palm leaves, a method that combines efficient techniques for grinding, homogenization and storage is presented. Good results were obtained for isozyme activity through starch gel electrophoresis for Malate dehydrogenase, Isocitrate dehydrogenase, Phosphogluco mutase, Phosphogluco isomerase and Acid phosphatase.

Keywords: Isozyme; Elaeis guineensis jacq.

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