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Identical isoenzyme patterns in sib plants of Relbunium hypocarpium(Rubiaceae)

Suzana Cavalli-Molina; Valmicir E.P. Motta; Marion Schiengold; Helga Winge

Departamento de Genética, Universidade Federal do Rio Grande do Sul, Caixa Postal 1953, 90001 Porto Alegre, RS, Brasil

ABSTRACT

Progenies of 9 plants of Relbunium hypocarpium (Rubiaceae) collected at different localities in Brazil and Argentina were studied for EST and/or PER patterns by polyacrylamide gel electrophoresis. From one strain, about 800 seedlings of 3 generations (F₁, F₂ and F₃) were analyzed for one (EST) or 4 enzyme systems: EST, PER, ACP and GOT. From this same strain, isoesterases of F₁ plants at different ontogenetic stages were also analyzed. In all cases, sibs at the same ontogenetic stage always showed identical isoenzyme patterns when the same tissue at the same stage of maturation was analyzed, showing absence of structural and also regulatory gene segregation. The results indicate that Relbunium hypocarpium is an autogamous species.

Keywords: isoenzyme; plants; Relbunium hypocarpium.

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