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Hormonal regulation of ribosomal RNA synthesis in immature rat uterus

C .H.B. Rossini^I; R.R. Brentani^II; N. Marquesa^III

^ILaboratório de Oncologia Experimental, Faculdade de Medicina da Universidade de São Paulo, São Paulo, S.P., Brasil. The present address of C.H.B.R. is Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Trindade. 88000 Florianópolis, S.C., Brasil
^IIInstituto Ludwig de Pesquisa sobre o Câncer, Rua Prof. Antonio Prudente, 109 - 4⁰. , 01509 São Paulo, SP, Brasil
^IIIDepartamento de Clínica Médica, Terapêutica I., Faculdade de Medicina, Universidade de São Paulo, 01246 São Paulo, SP, Brasil, and Grupo Multidisciplinar de Desenvolvimento e Ritmos Biológicos, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biomédicas, Universidade de São Paulo, 05508 São Paulo, SP, Brasil. Send correspondence to N.M. at the second address

ABSTRACT

Nuclear and nucleolar fractions from immature rat uterus injected with non-radioactive 17 -P-estradiol were isolated and characterized in terms of their morphological and biochemical properties. The fractions were essentially pure and transcription studies were carried out on isolated nuclei and nucleoli. Transcriptional activity of isolated nucleoli was only inhibited 20% by Cf-amanitin. Specific rat uterus estradiol receptors were determined in both the nuclear and nucleolar fractions by the exchange reaction after injecting rats with unlabeled 17-0-estradiol (and with competitive inhibition by diethylstilbestrol and saturation curves). The parameters for nuclear and nucleolar receptors, calculated from the dissociation constant derived from Scatchard plots of the binding curves, were Kd = 1.06 x 10^-9 M and 20.41 x 10^-8 M; Ka = 0.94 x 10¹⁰ M and 20.05 x 10⁹ M and Bo = 0.49 x 10^-9 M and 0.35 x 10^-9 M, respectively. The kinetic curve for the nucleoli suggested a late accumulation of the hormone-receptor complex, in contrast to the curve for the nuclei, which showed early accumulation of this complex.

Keywords: hormonal; regulation; ribosomal; synthesis; immature; rat; uterus.

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