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H-2 and t complexes in wild mice

Claudio Zúñiga; Sonia Fuenzalida; Olga Pizarro; Gladys Tapia; Ulises Vergara

Unidad de Biología y Genética, Div. Ciencias Médicas Occidente, Facultad de Medicina, Universidad de Chile, Casilla 33052 Correo 33, Santiago, Chile

ABSTRACT

Four t-haplotypes (t^Mo t^Ra, ^tLa and t^Qu) have been independently isolated from widely separated Chilean wild mouse populations. These t-haplotypes are now maintained in our laboratory as different tailless lines in combination with the brachyury chromosome (T). All the haplotypes are associated with a strong suppression of genetic recombination but they show different degrees of transmission ratio distortion. When serologically tested for the presence of H-2 antigenic determinants found in inbred strains, the t-bearing mice failed to react with most of the H-2 antisera but the combination of H-2 antigens expressed was sufficiently unique to differentiate each haplotype. The t^Ra lethal haplotype was antigenically characterized by the presence of the H-2D.2 private determinant and the t^MO viable haplotype was characterized by the presence of the H-2D.2 and the H-2K.19 private determinants. These results of the H-2 typing are in agreement with previous findings of close parenthood between the MHC of t-haplotypes and at least some classical MHC genes found in inbred strains.

Keywords: H-2; t complex; wild; mice.

REFERENCES

Bennett, D. (1975). The T-locus of the mouse. Cell 6:441-454.

Gorer, P.A. and Mikulska, Z.B. (1954). The antibodies response to tumor inoculation. Improved methods for antibody detection. Cancer Res. 14: 651-655.

Hammerberg, C. and Klein, J. (1975). Linkage disequilibrium between H-2 and t complexes in chromosome 17 of the mouse. Nature 258: 296-299.

Hansen, T.H., Spinella, D.G., Lee, D.R. and Shreffler, D.C. (1984). The immunogenetics of the mouse major histocompatibility gene complex. Ann. Rev. Genet. 18:99-129.

Hood, L., Steinmetz, M. and Malissen, B. (1983). Genes of the major histocompatibility complex of the mouse. Ann. Rev. Immunol. 1 :529-568.

Klein, J. and Hammerberg, C. (1977). The control of differentiation by the T complex. Immunol. Rev. 33:70-104.

Morita, T., Delarbre, C., Kress, M., Kourilsky, P. and Gachelin, G. (1985). An H-2K gene of the tw32 mutant at the T/t complex is closely related to H-2K^b or H-21( genes. Immunogenetics 21:367-383.

Nizetic, D., Figueroa, F. and Klein, J. (1984). Evolutionary relationships between the t and H-2 haplotypes in the house mouse. Immunogenetics 19: 311-320.

Parker, K.L., Atkinson, J.P., Roos, M.H. and Shreffler, D.C. (1980). Genetic and structural characterization of the H-2 controlled allelic forms of mucine C4. Immunogenetics 11:55-63.

Passmore, H.C. and Beisel, K.W. (1977). Preparation of antisera for the detection of Ss protein and the Slp alloantigen.Immunogenetics 4: 393-399.

Pizarro, O., Vergara, U. and Figueroa, F. (1977). A study of histocompatibility-2 antigens in wild mice from Santiago, Chile. Immunogenetics 4: 57-64.

Schwartz, R.H. (1985). T-lymphocyte recognition of antigen associated with gene products of the major histocompatibility complex. Ann. Rev. Immunol. 3: 237-261.

Shin, H.S., Stavnezer, J., Artzt, K. and Bennett, D. (1982). Genetic structure and origin of thaplotypes of mice analyzed with H-2-cDNA probes. Cell 29: 969-976.

Shreffler, D.C. (1976). The S region of the mouse histocompatibility complex (H-2): Genetic variation and functional role in complement system. Transplant. Rev. 32: 140-167.

Silver, L.M. (1982). Genomic analysis of the H-2 complex region associated with mouse t haplo- types. Cell 29: 961-968.

Silver, L.M. (1985). Mouse t haplotypes. Ann. Rev. Genet. 19: 179-208.

Silver, L.M. and Artzt, K. (1981). Recombination suppression of mouse t haplotypes due to chromatin mismatching. Nature 290: 68-70.

Vergara, U. (1983). T/t and H-2 systems in wild mice. II. t haplotypes and SsSlp proteins. Braz. J. Genet. 6: 229-235.

Weeke, B. (1973). Rocket immunoelectrophoresis. In: A manual of quantitative immunoelectrophoresis. (Axelsen, N.H., Kroll, J. and Weeke, B., eds.). Universitets Forlaget, Oslo, pp. 36-46.

Zinkernagel, R.M. and Doherty, P.C. (1979). MHC restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction- specificity, function and responsiveness. Adv. Immunol. 27: 51-177.