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The isolation of a 20kb transposon, tn4527, carrying resistance to trimethoprim, spectinomycin and streptomycin

Ana Teresa Nogueira Dumans; Ana Maria Abrantes Coelho

Instituto de Biologia da Universidade Federal do Rio de Janeiro, Caixa Postal 68.011, Ilha do Fun-dio, 21941 Rio de Janeiro, RJ, Brasil

ABSTRACT

Tn4527 was isolated from a Salmonella typhimurium strain obtained in Brazil. Its size is approximately 20 kb, being one of the largest antibiotic resistance transposons isolated from Enterobacteria. It carries resistance to trimethoprim , spectinomycin and streptomycin. It transposed originally to R64drd11 and from this plasmid to pDS4161, a multicopy Cm^r ColK derivative.
Cm^s derivatives were obtained from pDS4161: : Tn4527. The Cm^s isolates have lost a 1.6kb Pst I fragment, including DNA from the pDS4161.
The Tp resistance carried by Tn4527 is not expressed in one of the strains used (FG 151). 1 Tp: trimethoprim; Sp: spectinomycin; Sm: streptomycin; Cm: chloramphenicol; Km: kanamycin; Ap: ampicillin; Tc: tetracycline.

Keywords: transposon; carrying; resistance; trimethoprim; spectinomycin; streptomycin.

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