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A simple method for fresh-water fish lymphocyte culture

Alberto Sérgio Fenocchio^I; Luiz A.C. Bertollo^II

^IUniversidade Nacional de Misiones, Misiones, Argentina
^IIDepartamento de Ciências Biológicas, Universidade Federal de São Carlos, 13560 Ho Carlos, SP, Brasil. Send correspondence to L.A.C.B.

ABSTRACT

We describe a technique of whole peripheral blood lymphocyte culture for application in studies of fish cytogenetics. This method is relatively simple, shows high repeatability and produces good-quality chromosome preparations.

Keywords: method; fresh-water; fish; lymphocyte.

REFERENCES

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Kligerman, A.D. and Bloom, S.E. (1977). Rapid chromosome preparations from solid tissues of fishes. J. Fish. Res. Board Can. 34 : 266-269.