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Cytogenetic analysis of human meningiomas

Silvia Regina Rogatto^I; Cacilda Casartelli^II

^IDepartamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86051 Londrina, PR, Brasil
^IIDepartamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049 Ribeirão Preto, SP, Brasil. Send correspondence to C.C.

ABSTRACT

Four human primary meningiomas were cultured and analyzed cytogenetically: 1 transitional meningioma with a modal chromosome number of 46 (I), 2 fibroblastic meningiomas both with a modal chromosome number of 45 (II and III) and 1 meningoendotheliomatous meningioma with a modal class of 781-82 chromosomes (IV). The most consistent alteration detected in all tumors was total or partial monosomy of chromosome 22 (I - 30%; II - 25%; III - 69%, and IV - 30% of metaphases). Tumor I also showed loss of the Y chromosome (80% of the cells) in addition to other unidentified markers. Tumor II showed the deletions 12p12 ---> 12pter (65% of cells) and del(19) (qter - p13:) (50% of the cells). Tumor III showed 3 recurrent markers that were not identified. Tumor IV showed a large acrocentric marker that was not identified. Sporadic monosomies, trisomies and tetrasomies were also detected in these tumors.The recurrent breakpoints were compared with fragile sites, specific breakpoints in neoplasms, active genes of. differentiated cells, and oncogenes described in the literature

Keywords: cytogenetic; human; meningiomas.

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