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Ethyl methanesulfonate-induced mutations in Phaseolus vulgaris l.

Hélio M. Barbosa^I; José Eustáquio de S. Carneiro^II; Clibas Vieira^III

^IDepartamento de Biologia Geral, Universidade Federal de Viçosa, 36570 Viçosa, MG, Brasil. Send correspondence to H.M.B.
^IIEMBRAPA/CNPAF, 74000 Goiânia, GO, Brasil
^IIIDepartamento de Fitotecnia, Universidade Federal de Viçosa, 36570 Viçosa, MG, Brasil

ABSTRACT

Germinating seeds of Phaseolus vulgaris L. were treated with 0 (control), 0.0625, 0.125, 0.25, and 0.5% ethyl methanesulfonate (EMS) solutions. Hypocotyl and flower color mutations were detected in the M₂ generation mostly after 0.25% EMS treatment. The relative success of selecting for seedcoat color mutants based on the correlation between hypocotyl color and seedcoat color is discussed. For each mutagen concentration the frequency of chlorophyll deficiency mutations of the types albina, xantha and viridis increased in that order. Several morphologic mutants were found. A few of these recovered from the anomaly during later onto-genetic stages and may turn out to be good markers for genetic studies.

Keywords: ethyl methanesulfonate; mutations; Phaseolus vulgaris.

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