Full text in pdf format

Cytogenetic analysis of 39 bovine embryos obtained from superovulated females

Irineu M. Benevides-Filho^I; Luiz E. L. Pinheiro^II

^IDepartamento de Zootecnia, Faculdade de Veterinária, Universidade Federal Fluminense, Caixa Postal 100.086, 24230 Niterói, RJ, Brasil. Send correspondence to I.M.B.-F.
^IIDepartamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais

ABSTRACT

A study was conducted on 163 bovine embryos aged 7 days and on 29 nonfertilized structures obtained from cows of several breeds treated with FSH for superovulation. Of the total number of embryos processed, 87 (53.37%) showed metaphases. The chromosome number was determined for 39 of these (44.83%) and found to be normal for the species in 25 (64.10%). The remaining 14 (35.90%) showed numerical alterations such as haploidy (N = 1), hypodiploidy (N = 4), hyperdiploidy (N = 4), and mixoploidy (N = 5). The sex chromosomes were identified in 29 (74.36%) embryos.

Keywords: cytogenetic; bovine; embryos; superovulated; females.

REFERENCES

Ayalon, N. (1968). A review of embryonic mortality in cattle. J. Reprod. Fertil. 54: 483-493.

Diskin, M.G. and Sreenan, J.M. (1980). Fertilization and embryonic mortality rates in beef heifers after artificial insemination. J. Reprod. Fertil. 59:463-468.

Gayerle de Abreu, F., Lamming, G.E. and Shaw, R.C. (1984). A cytogenetic investigation of early stage bovine embryos in relation with embryo mortality. Proc. Int. Congr. Anim. Reprod. and Art. Insem., 10, Urbana, I11, Vol. 2, p. 82.

Greenstein, J.S., Murray, R.W. and Foley, R.C. (1958). Observations on the morphogenesis and histochemistry of the bovine pre-attachment placenta between 16 and 33 days of gestation. Anat. Rec. 132: 321-341.

Jacobs, P.A. (1982). Pregnancy losses and birth defects. In: Embryonic and Fetal Development (Austin, C.R. and Short, R.V., eds.). (Reproduction in Mammals, 2). 2nd edn. Cambridge University Press, Cambridge, pp. 142-158.

Hare, W.C.D. and Singh, E.L. (1980). Chromosomal analysis of early bovine embryos. Eur. Coll. Cytogent. Dom. Anim., 4, Uppsala, Sweden, pp. 172-180.

Hunter, R.H.F. (1985). Fertility in cattle: Basic reason why late insemination must be avoided. Anim. Breed. Abstracts 53: 83-87.

King, W.A. (1980). Spontaneous chromosome translocations in cattle and pigs: A study of the causes of their fertility reducing effect. Ph.D. Thesis. Swedish University of Agricultural Sciences, 56 pp.

King, W.A. (1983). Cytogenetics of embryos: Application to the livestock industry. Symp. Adv. Top. Anim. Reprod., 2, Jaboticabal, UNESP, pp. 113-132.

King, W.A., Linares, T., Gusta^yson, I. and Bane, A. (1979). A method for preparation of chromosomes for bovine zygotes and blastocysts. Vet. Sci. Commun. 3:51-56.

King, W.A., Linares, T. and Gusta^yson, I. (1981). Cytogenetics of preimplantation embryos sired by bulls heterozygous for the 1/29 translocation. Hereditas 94: 219-224.

Long, S.E. and Williams, C.V. (1980). Frequency of chromosomal abnormalities in early embryos of domestic sheep. J. Reprod. Fertil. 58: 197-201.

Maudlin, I. and Fraser, L.R. (1977). The effect of PMSG dose on the incidence of chromosomal anomalies in mouse embryos fertilized in vitro. J. Reprod. Fertil. 50: 275-280.

Murray, J.D., Moran, C., Boland, M.P., Doff, A.M. and Nancarrow, C.D. (1985). Cytogenetic analysis of 34 early stage bovine embryos from a superovulated Hereford donor. Can. J. Genet. Cytol. 27:483-486.

Staples, R.W., McEntee, K. and Hansel, W. (1969). Luteal function as related to pituitary and ovarian cytology and embryo development. J. Dairy Sci. 44: 2049-2057.

Tarkowiski, A.K. (1966). An air-drying method for chromosome preparations from mouse eggs. Cytogenetics 5: 349-400.