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Selection of D. melanogaster for extreme developmental rates. I. Structural and regulatory genetic divergence

Elgion L.S. Loreto; Alice Kalisz de Oliveira

Departamento de Genética, Universidade Federal do Rio Grande do Sul, Caixa Postal 1953, 90001 Porto Alegre, RS, Brasil. Send correspondence to A.K.L. The present address of E.L.S.L. is Departamento de Metodologia do Ensino, Universidade Federal de Santa Maria, 97100 Santa Maria, RS, Brasil

ABSTRACT

Electrophoretic analysis of 5 enzyme systems for a total of 12 loci in different developmental stages of D. melanogaster populations selected over 485 generations for fast (early) development and over 335 generations for slow (late) development, and of a control population showed structural genetic similarity, as measured by allele substitutions, which was 0.92 between control and late populations, 0.83 between control and early populations, and 0.75 between early and late populations. Regulatory similarity measured by the Jaccard index was 0.947 between control and early populations, 0.940 between control and late populations, and 0.908 between early and late populations. Similarity calculated by the Pearson coefficient was 0.949 between control and early populations, 0.940 between control and late populations, and 0.894 between early and late populations. It is suggested that divergence in class of regulatory genes may be of great importance in the speciation process.

Keywords: D. melanogaster; developmental; structural; regulatory; genetic; divergence.

REFERENCES

Avise, J.C. (1976). Genetic differentiation during speciation. In:Molecular Evolution (Ayala, F.J., ed.). Sinauer, Sunderland, pp. 106-122.

Ayala, F.J. (1980). Speciation: stages, modes and genetical- analisis - Simposia. Univ. Simon

Bolivar. Ecologia e genética de la especiación animal. Editora da Univ. Simon Bolivar.

Ayala, F.J., Tracey, M.L., Hedgecock, D. and Richmond, R. (1974). Genetic differentiation during the speciation process in Drosophila. Evolution 28: 576-592.

Bewley, G.C. and Lucchesi, J.C. (1977). Origin of a-glycerophosphate dehydrogenase isozymes in D. melanogaster and their functional relationship in the «-glycerophosphate cycle. Biochem. Genet. 15: 235-251.

Dickinson, W.J. (1980). Evolution of patterns of gene expression in Hawaiian picture-winged Drosophila. J. Mot. Evol. 16:73-94.

Fox, D.J. (1971). The soluble citric acid cycle enzymes of D. melanogaster. I. Genetics and ontogeny of NADP-linked isocitrate dehydrogenase. Biochem. Genet. 5: 69-80.

Hedrick, P.W. and McDonald, J.F. (1980). Regulatory gene adaptation: an evolutionary model. Heredity 45: 83-97.

Huettel, R.N. and Bush, G.L. (1972). Starch Gel Electrophoresis of Tephritid Proteins. A Manual of Techniques. Groups on Fruits Flies. International Biological Programme Press, Austin.

Jacobson, K.B., Murphy, J.B., Knopp, J.A. and Ortiz, J.R. (1972). Multiple forms of Drosophila alcohol dehydrogenase. III. Conversion of one form to another by NAD or acetone. Arch. >Biochem. Biophys. 149: 22-35.

Kimura, M. (1968). Evolutionary rate at the molecular level. Nature 217: 624-626.

King, M.C. and Wilson, A.C. (1975). Evolution at two levels. Molecular similarities and biological differences between humans and chimpanzees. Science 188: 107-116.

Loreto, E.L.S. and Oliveira, A.K. (1988). Epigenetic changes of IDH-NADP isoenzymes in several Drosophila species. Rev. Bras. Genet. 11 (in press).

McDonald, J.F., Chambers, G.K., David, J. and Ayala, F.J. (1977). Adaptive responses due to changes in gene regulation: a study with Drosophila. Proc. Natl. Acad. Sci., USA 74: 45624566.

McDonald, J.F. and Ayala, F.J. (1978). Gene regulation in adaptive evolution. Can. J. Genet. Cytol. 20: 159-175.

Napp, M. and Brncic, D. (1978). Electrophoretic variability in two closely related Brazilian species of the flavopilosa species group of Drosophila. Rev. Bras. Genet. 1: 1-10.

Oliveira, A.K. (1979). Controle genético da velocidade de desenvolvimento e dos padrdes de proteínas na ontogenia de D. melanogaster. Doctoral Thesis. UFRGS, Porto Alegre.

Oliveira, A.K. and Cordeiro, A.R. (1981). Genetic control of developmental rate and a mother factor in D. melanogaster. Rev. Bras. Biol. 41: 635-644.

Poulik, M.D. (1957). Starch gel electrophoresis in a discontinuous system of buffers. Nature 180: 1477-1479.

Sene, F.M. and Carson, H.L. (1977). Genetic variation in Hawaiian Drosophila. IV. Allozymic similarity between D. silvestris and D. heteroneura from the island of Hawaii. Genetics 86: 187-198.

Sneath, P.H.H. and Sokal, R.R. (1973). Numerical Taxonomy. W.H. Freeman, São Francisco, pp. 114-187.

Wilson, A.C. (1976). Gene regulation in evolution. In: Molecular Evolution (Ayala, F.J. ed.). Sinauer, pp. 225-236.