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Isolation and characterization of drug-resistant mutants of Herpetomonas samuelpessoai (Kinetoplastida, Trypanosomatidae)

Wanda M.A. Von Krüger; Amilcar Tanuri; D.F. de Almeida

Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21941 Rio de Janeiro, RJ, Brasil. Send correspondence to D.F. de Almeida

ABSTRACT

Fifteen drugs were tested against wild-type cells of Herpetomonas samuelpessoai and their minimum inhibitory concentrations (m.i.c.) determined. They could be grouped in three classes on the basis of their minimal inhibitory concentration values: those 1) only inhibiting cell growth at relatively high concentrations (> 250 ,i1g/ml); 2) displaying moderate m.i.c. values; and 3) toxic at very low concentrations (<5 /lg/ml). After a) mutagenesis by U.V., nitrosoguanidine or ethylmethane sulfonate, b) selection in a stepwise manner for high levels of resistance to drugs and c) spontaneous selection, drug-resistant isolates were obtained. These included unstable phenotypes and stable mutants. The latter were partially characterized and their properties suggest they will be suitable to study genetic recombination in Herpetomonas samuelpessoai.

Keywords: characterization; drug-resistant; mutants; Herpetomonas samuelpessoai.

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