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Iodo-gen-catalyzed iodination for identification of surface-exposed outer membrane proteins of Escherichia coli K12

L.C.S. Ferreira^I; Darcy F. de Almeida^II

^IDepartamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, CCB, 50000 Recife, PE, Brasil. Send correspondence to L.C.S.F.
^IIInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS, 21941 Rio de Janeiro, RJ, Brasil

ABSTRACT

Surface proteins of Escherichia coli K12 were identified by radiolabelling using 1,3,4,6tetrachloro,3-alpha,6-alpha-diphenylglycoluryl (Iodo-Gen) and ¹³¹I. Labelled proteins were localized in the outer membrane of the cells. Using this technique it has been possible to observe that the eletrophoretic pattern of surface proteins changes according to the growth phases in culture. Radiolabelling of E. coli cells incubated at 42°C showed that the syntheses of two surface proteins were temperature-inducible. At least one such protein may be involved in the process of cell division in E. coli K12.

Keywords: iodo-gen-catalyzed; iodination; surface-exposed; membrane; proteins; Escherichia coli .

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