Full text in pdf format
The contrabithorax mutant protein products
Eliana Abdelhay; Humberto Rocha
Laboratório de Biologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21941 Rio de Janeiro, RJ, Brasil. Send correspondence to E.A.
ABSTRACT
The meso- and metathoracic imaginal discs protein pattern in D. melanogaster wild type and Contrabithorax (Cbx) mutant were analyzed by two-dimensional gel electrophoresis. Some qualitative differences were detected between the wild type and Cbx mesothoracic pattern.
Keywords: contrabithorax; mutant; protein.
REFERENCES
Akam, M. (1983). Decoding the Drosophila complexes. TIBS 8: 173-177.
Akam, M.E. (1983). The location of ultrabithorax transcripts in Drosophila tissue sections. EMBO J. 2: 2075-2084.
Akam, M. and Martinez-Arias, A. (1985). The distribution of ultrabithorax transcripts in Drosophila embryos. EMBO J. 4:1689-1700.
Ames, G.F.L. and Nikaido, K. (1976). Two dimensional gel electrophoresis of membrane proteins. Biochemistry 15:616-623.
Beachy, P.A., Helfand, S.L. and Hogness, D.S. (1985). Segmental distribution of bithorax complex proteins during Drosophila development. Nature 313: 545-551.
Bender, W., Akam, M., Karch, F., Beachy, P.A., Peifer, M., Spierer, P., Lewis, E.B. and Hogness, D. (1983). Molecular genetics of bithorax complex in Drosophila melanogaster. Science 221: 23-29.
Bonner, W.M. and Laskey, R.P. (1974). A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46: 83-88.
Casanova, J., Sanchez Herrero, E. and Morata, G. (1985). Contrabithorax and the control of spatial expression of the bithorax complex genes of Drosophila. J. Embryol. Exp. MorphoL 90: 179196.
Chan, L.N. and Gehring, W. (1971). Determination of blastoderm cells in Drosophila melanogaster. Proc. NatL Acad. Sci. WA. 68: 2217-2221.
Dewey, M.S., Filler, R. and Mintz, B. (1978). Protein patterns of developmentally totipotent mouse teratocarcinoma cells and normal embryo cells. Dev. BioL 65: 171-182.
Echalier, G. and Ohanessian, A. (1970). In vitro culture of Drosophila melanogaster embryonic cells. In Vitro 6: 162-172.
Fjose, A., McGuinnis, W.S. and Gehring, W.J. (1985). Isolation of a homeo-box-containing gene from the engrailed region of Drosophila and the spatial distribution of its transcripts. Nature 313: 284-289.
Garcia-Bellido, A. (1977). Homeotic and atavic muta tions in insects. Am. Zool. 17: 613-629.
Greenberg, R.M. and Adler, P.N. (1982). Protein synthesis and accumulation in Drosophila melanogaster imaginal discs: identification of a protein with a nonrandom spatial distribution. Dev. Biol. 89: 273-286.
Gruenstein, E. and Pollard, A.L. (1976). Double label autoradiography on polyacrylamide gels with H3 and C14. Anal. Biochem. 76:452-457.
Karch, F., Weiffenbach, B., Peifer, M., Bender, W., Duncan, I., Celniker, S., Crosby, M. and Lewis, E.B. (1985). The abdominal region of the bithorax complex. Cell 43: 81-96.
Kornberg, T., Siden, I., O'Farrel, P. and Simon, M. (1985). The engrailed locus of Drosophila: in situ localization of transcripts reveals compartment specific expression. Cell 40:40-53.
Laemmli, U.K. (1970). Cleavage of structural proteins during assembly of the head of bacterio- phage T4. Nature 223: 680-685.
Lewis, E.B. (1978). A gene complex controlling segmentation in Drosophila. Nature 276: 565570.
Lawrence, P.A. and Morata, G. (1983). The elements of the bithorax complex. Cell 35: 595-01.
McGuinnis, W., Levine, M.S., Hafen, E., Kuroiwa, A. and Gehring, W. (1984). A conserved DNA sequence in homeotic genes of Drosophila antennapedia and bithorax complexes. Nature 308:428-433.
Morata, G. (1975). Analysis of gene expression during development in the homeotic mutant Contrabithorax of Drosophila melanogaster. J. Embryo!. Exp. Morphol. 34: 19-31.
Morata, G., Sanchez Herrero, E. and Casanova, J. (1986). The bithorax complex of Drosophila: an overview. Cell Differ. 18:67-78.
Morata, G. and Kerridge, S. (1981). Sequential functions of the bithorax complex. Nature 290: 778-781.
O'Farrell, P. (1975). High resolution two dimensional electrophoresis of proteins. J BioL Chem. 250:4007-4021.
Regulski, M., Harding, K., Kostriken, R., Karch, F., Levine, M. and McGuinnis, W. (1985). Homeo Box genes of the antennapedia and bithorax complexes of Drosophila. Cell 43: 71-80.
Sanchez-Herrero, E., Vernon, I., Marco, R. and Morata, G. (1985). Genetic organization of Drosophila bithorax complex. Nature 313: 108-113.
Scott, M.P., Weiner, A.J., Hazerigg, T.I., Polinsky, B.A., Pirrotta, V., Scalenghe, F. and Kaufman, T.C. (1983). The molecular organization of the antennapedia locus of Drosophila. Cell 35: 763-776.
Wieschaus, E. and Gehring, W. (1976). Clonal analysis of primordial disc cells in the early embryo of Drosophila melanogaster. Dev. Biol. 50: 249-263.
White, R.A. and Wilcox, M. (1984). Protein products of the bithorax complex in Drosophila. Cell 39: 163-171.
White, R.A.H. and Wilcox, M. (1985a). Distribution of ultrabithorax proteins in Drosophila. EMBO J. 4: 2035-2043.
White, R.A.H. and Wilcox, M. (1956b). Regulation of the dist ribution of ultrabithorax proteins in Drosophila. Nature 318: 563-567.