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The ultrastructure of the larval hemocytes of Trichosia pubescens (Diptera: Sciaridae)

Margherita A. Barracco^I; Antonio N. Cestari^II

^IDepartamento de Biologia, Universidade Federal de Santa Catarina, Caixa Postal 476, 88049 Florianópolis, SC, Brasil
^IIDepartamento de Biologia, Instituto de Biociências, Universidade de São Paulo, Caixa Postal 11461, 05499 São Paulo, SP, Brasil. Send correspondence to A.N.C.

ABSTRACT

Four hemocyte types were identified by transmission electron microscopy in the hemolymph of Trichosia pubescens: 1) Prohemocytes, small cells whose nucleus fills the cytoplasm almost entirely; the thin cytoplasm layer is mainly characterized by a large amount of loose ribosomes and a poorly developed membranous system; 2) Plasmocytes, polymorphic hemocytes which are very rich in rough endoplasmic reticulum, showing different degrees of cisternal dilatation and presenting a large number of acid phosphatase positive lysosomes; 3) Oenocytoids, the largest hemocytes which are mainly characterized by a wide cytoplasm layer strikingly poor in membranous systems; they contain a large amount of free ribosomes; 4) Vacuolated cells, ununsual hemocytes which present an extremely vacuolated cytoplasm. This cytoplasm seems to be invaded by many channels originating from plasma membrane invaginations. They also present rough endoplasmic reticulum, Golgi bodies and mitochondria.

Keywords: ultrastructure; hemocytes; Trichosia pubescens.

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