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Satellite DNA in species of the genus Oryzomys (Rodentia, Cricetidae)

Lidia Vidal-Rioja; Daniel Corach; Juan Padrón

Departamento de Citogenética Molecular, IMBICE, Casilla Postal 403, 1900 La Plata, Argentina. Send correspondence to L.V.-R.

ABSTRACT

The DNA from cricetid rodents of the genus Oryzomys (O. flavescens, O. longicaudatus longicaudatus, and O. 1. magellanicus) was investigated. CsC1 gradient centrifugations were performed with total DNA, total DNA combined with the dye Hoechst 33258 and with fast reassociated DNA isolated from hydroxyapatite columns. Thermal denaturations were carried out on total native DNA. In the three species some 7%; of total DNA appeared as a highly repetitive satellite rich in A-T bases. In each species, bulk DNA and satellite melted as two different thermal components. First derivative analysis of the melting profiles showed minor differences in the Tm and melting transition intervals of the minor components. These differences were statistically significant between O. flavescens and each O. longicaudatus and non-significant between the two subspecies of O. longicaudatus. Compositional features of the satellite would suggest a common origin prior to the divergence of the three Oryzomys forms. It is assumed that along with the chromosomal diversification and geographical isolation of the species some sequence divergence of the satellite DNAs may have also occurred.

Keywords: satellite DNA; Oryzomys.

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