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Effect of acridine orange and acriflavine on fertility in Drosophila melanogaster and D. simulans

 

 

R. Marcos; P. Alba; N. Xamena; A. Creus

Divisió de Genètica, Departament de Genètica i Microbiologia, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. Send correspondence to R.M.

 

 


ABSTRACT

Acridine orange (AO) and acriflavine (AF) were fed to adult Drosophila melanogaster and D. simulans in order to test their effects on fertility. The two compounds produced a significant decrease in the fertility of both species, the effect of AO being more pronounced. Furthermore, D. simulans seems to be more sensitive.

Keywords: acridine orange; acriflavine; fertility; Drosophila melanogaster; D.simulans.


 

 

REFERENCES

Alba, P. (1983). Genotoxicidad del naranja de acridina y de la acriflavina en Drosophila. "Licenciatura" Thesis, Universidad Autónoma de Barcelona, 118 pp.

Alba, P., Ferrés, M.D., Xamena, N., Creus, A. and Marcos, R. (1983a). Differences between Drosophila melanogaster and its sibling species D. simulans in sensitivity to acridine orange treatment. Experientia 37: 300-301.

Alba, P., Ferrés, M.D., Xamena, N., Creus, A. and Marcos, R. (1983b). Genotoxicity of acridine orange and acriflavine in Drosophila melanogaster. Mutat. Res. 121: 199-203.

Albert, A. (1966). The A cridines, their Preparation, Physical, Chemical, and Biological Properties and Uses, 2nd edition. Arnold, London, pp. 604.

Barker, G.R. and Hardman, N. (1974). Growth inhibition of Escherichia coli by acridine orange and its dependence on pH. Biochem. Soc. Trans. 2: 330-333.

Bateman, A.J. and Epstein, S.S. (1971). Dominant lethal mutations in mammals. In: Chemical Mutagens (A. Hollaender, Ed.), Vol. 2. Plenum Press, New York, pp. 541-568.

Bernheim, N.J. and Falk, H. (1981). Chemical, physical and genetic factors interfering with DNA repair – A review. J. Am. CoiL Toxicol. 2: 23-54.

Hirota, Y. (1960). The effect of acridine dyes on mating type factors in Escherichia coli. Proc. Natl. Acad. Sci. USA 46: 57-64.

Hirota, Y. and lijima, T. (1957). Acriflavine as an effective agent for eliminating F-factor in Escherichia coli K12. Nature 180: 655-656.

Lasnitzki, I. and Wilkinson, J.H. (1948). The effect of acridine derivatives on growth and mitosis of cells in vitro. Br. J. Cancer 11: 369-375.

Marcos, R., López de Sepúlveda, J., Xamena, N. and Creus, A. (1981). Effect of ethidium bromide on Drosophila melanogaster and D. simulans. Experientia 37: 559-560.

Nasim, A. and Brychcy, T. (1979). Genetic effects of acridine compounds. Mutat. Res. 65: 261288.

Parsons, P.A. (1975). Differences between Drosophila melanogaster and its sibling species D. simulans to radioresistance using isofemale strains from natural populations. Int. J. Radiat. Biol. 27: 297-300.

Parsons, P.A. (1979). Resistance of the sibling species Drosophila melanogaster and Drosophila simulans to high temperatures in relation to humidity: Evolutionary implications. Evolution 33:131-136.

Ross, W.E. and Bradley, M.O. (1981). DNA double-strand breaks in mammalian cells after exposure to intercalating agents. Biochim. Biophys. Acta 654: 121-134.

Vogel, E. and Sobels, F.H. (1976). The function of Drosophila in genetic toxicology testing. In: Chemical Mutagens (A. Hollaender, Ed.), Vol. 4. Plenum Press, New York, pp. 93-142.

Vogel, E. and Lüers, H. (1974). A comparison of adult feeding to injection in Drosophila melanogaster. Drosophila Inf. Serv. 51: 113-114.

Xamena, N., Creus, A. and Marcos, R. (1984). Mutagenic activity of some intercalating compounds in the Drosophila zeste somatic eye mutation test. Mutai. Res. 138: 169-173.

Yamagata, H. and Uchida, H. (1969). Effect of acridine orange on sex-factor multiplication in Escherichia coli. J. Mol. Biol. 46:73-84.