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Effect of radiation on the female germ cells of Drosophila melanogaster strains

M.S. Benfato; H.H.R. Andrade

Departamento de Genética, Universidade Federal d0o Rio Grande do Sul, Caixa Postal 1953, 90000 Porto Alegre, RS, Brasil. Send correspondence to H.H.R.A.

ABSTRACT

CO₃ and RC₁ strains of Drosophila melanogaster were investigated for differences in sensitivity to the induction of dominant lethals during two different stages of oogenesis, i.e. stage-7 and stage-14 oocytes. For the study of stage-7 oocytes, CO₃ and RC₁ females were irradiated with 1.25, 2.5, 3.75, 5 and 10 KR of ⁶⁰Co gamma radiation. The doses applied to stage-14 oocytes were 0.125, 0.35, 0.375, 0.5 and 0.625 KR.
An experiment involving fractionated doses was performed on stage-7 oocytes, when 2.5 and 5 KR doses were applied as an unfractionated dose or subdivided into two equal fractions separated by a 30-minute interval.
The results suggest that: 1) at stage 7, strain CO₃ was resistant when compared to strain' RC ₁;2) at stage 14, on the other hand, strain RC ₁showed resistance to the 0.375, 0.5 and 0.625 doses, whereas no difference occurred between strains at the 0.25 KR dose, and the CO3 strain was resistant when compared to the RC₁ strain at the 0.125 KR dose; 3) the fractionated dose experiment showed that the RC₁ strain is defective in one of the phases of excision repair that follow the action of endonuclease; 4) the differences in sensitivity between CO₃ and RC₁ at stage 7 seem to be due to differences in repair, and the differences at stage 14 are due to selective elimination of the most sensitive cells from the mutability viewpoint, as well as to differential viability.

Keywords: radiation; germ cells; Drosophila melanogaster.

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